Methylated DNA Immunoprecipitation
DNA methylation, 5-Methylcytosine, Antibody
978-620-0-95085-7
6200950857
96
2012-03-19
34.00 €
eng
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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Methylated DNA immunoprecipitation is a large-scale purification technique in molecular biology that is used to enrich for methylated DNA sequences. It consists of isolating methylated DNA fragments via an antibody raised against 5-methylcytosine. This technique was first described by Weber M. et al. and has helped pave the way for viable methylome-level assessment efforts, as the purified fraction of methylated DNA can be input to high-throughput DNA detection methods such as high-resolution DNA microarrays or next-generation sequencing. Nonetheless, understanding of the methylome remains rudimentary; its study is complicated by the fact that, like other epigenetic properties, patterns vary from cell-type to cell-type.
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