Genetic engineering
principle of molecular biology and genetic engineering
978-3-8383-7568-7
3838375688
100
2010-07-13
49.00 €
eng
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This book covers the following topics: Recombinant DNA technology Restriction endonucleases T4 DNA ligase Plasmid cloning vectors pBR322 plasmid pUC19 plasmid Bacteriophage λ vectors Cosmids Transformation and selection Cloning DNA sequences that encode eukaryotic protein Synthesis of cDNA Creating and Screening a library Library construction methods Random mutagenesis methods Recombination-based methods Homology-dependent methods Homology-independent methods Targeted mutagenesis methods Selection/screening for desired property Genotype-phenotype linkage DNA sequencing techniques PCR Protein engineering Directed evolution Applications of directed evolution tool Rational design and site directed mutagenesis
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Biochemistry, biophysics
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