Bookcover of Functions of Transcription Factor Alpha-Pal/NRF-1 in Neurons
Booktitle:

Functions of Transcription Factor Alpha-Pal/NRF-1 in Neurons

Alpha-Pal/NRF-1 regulates IAP/CD47 Expression and Neurite Outgrowth in Neurons

VDM Verlag Dr. MŸller Aktiengesellschaft & Co. KG (2008-05-05 )

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ISBN-13:

978-3-8364-9683-4

ISBN-10:
3836496836
EAN:
9783836496834
Book language:
English
Blurb/Shorttext:
This book adopts modern molecular biology techniques to provide evidence demonstrating the transcription factor alpha-Pal/NRF-1 is a critical regulator of the human IAP/CD47 gene. This book provides evidence to demonstrate a novel function of alpha-Pal/NRF-1 in regulation of neurite outgrowth in neurons and this function is mediated by its downstream gene IAP/CD47. This book describes practical tips for promoter studies and protein functions in neurons, such as RNA isolation, reverse-transcription-polymerase chain reaction, site-directed mutagenesis, deletion constructs, electrophoretic mobility shift and supershift assays, luciferase assays, isolation of stable cell lines, primary neuronal cultures, transient transfection, immunofluorescence microscopy, and measurements of neurite outgrowth. Therefore, this is a reference book for molecular neuroscientists to study neuronal genes and functions.
Publishing house:
VDM Verlag Dr. MŸller Aktiengesellschaft & Co. KG
By (author) :
Wen-Teng Chang , A-Min Huang
Number of pages:
92
Published on:
2008-05-05
Stock:
Available
Category:
Chemistry
Price:
49.00 €
Keywords:
NRF-1, IAP/CD47, Gene Regulation, neurite outgrowth, promoter assay, human neuroblastoma IMR-32

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