Bookcover of Carica papaya lipase: a versatile enantioselectivity biocatalyst
Booktitle:

Carica papaya lipase: a versatile enantioselectivity biocatalyst

Applications of medium engineering and substrate engineering to improving Carica papaya lipase- catalyzed kinetic resolution of (R,S)-naproxen esters

LAP LAMBERT Academic Publishing (2011-03-10 )

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ISBN-13:

978-3-8443-1730-5

ISBN-10:
3844317309
EAN:
9783844317305
Book language:
English
Blurb/Shorttext:
The papaya lipase stored in the crude papain from spay-dried latex of Carica papaya has been discovered as a versatile enantioselectivity biocatalyst to obtain chiral acids by using hydrolytic resolution of their racemic esters. With adopting the enzyme screening, purification, medium engineering and substrate engineering tactics can effectively improve the ability of lipase- catalyzed hydrolysis of (R,S)-naproxen ester. By employing partially purified lipase or an appropriate organo-soluble base (ex: Triethylamine) addition in water-saturated isooctane, both have superior enhancement of lipase activity or enantioselectivity. In addition, it was also found that initial rate enhancement for (S)-ester displayed as increasing the inductive parameter (electron-withdraw capability), therefore it further implies the acylaction step must be the rate- limiting step during enzyme-catalyzed process. Besides, a novel means of applying substrate- assisted catalysis demonstrated the proton shuttle device acts as an effective tool for improving the lipase activity by forming an extra intra-molecular hydrogen bond (11.5~11.9 KJ/mol) in the transition state.
Publishing house:
LAP LAMBERT Academic Publishing
Website:
https://www.lap-publishing.com/
By (author) :
Jim Chen, Shau-Wei Tsai
Number of pages:
160
Published on:
2011-03-10
Stock:
Available
Category:
Chemical technology
Price:
59.00 €
Keywords:
Carica papaya lipase, (R, S)-naproxen 2, 2, 2-trifluoroethyl ester, kinetic resolution, organo-soluble base, substrate-assisted catalysis., R

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